Composite

Part:BBa_K5062017:Design

Designed by: Christian Nelson Tejedor Vicera   Group: iGEM24_HKU-HongKong   (2024-09-27)


Anti-PD1 Short Hairpin RNA (shRNA) Release System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 81
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This can be placed between any RNA polymerase and terminator. However, since it is not a protein coding domain it must not be placed in or disrupt any open reading frame as it will disrupt protein expression or be disrupted by protein expression.


Source

This sequence is either designed manually, generated using a tool from VectorBuilder, or found on a ribozyme database

References